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manA [2019-06-21 12:20:31]
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manA [2019-06-21 12:20:31]

mannose-6-phosphate isomerase, required for proper cell wall synthesis
locus
BSU12020
pI
5.28
mw
35.85 kDa
protein length
315 aa Sequence Blast
gene length
948 bp Sequence Blast
function
mannose utilization
product
mannose-6-phosphate isomerase
essential
no
synonyms
yjdE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.9|Utilization of mannose]

    • Gene

    Coordinates
    1,274,692 → 1,275,639

    Phenotypes of a mutant

  • cells lose the characteristic rod shape typical of wild type ''B. subtilis'' cells and instead appear as elongated spheres, which are significantly larger than normal [Pubmed|20862359]

    • The protein

    Paralogous protein(s)

  • [protein|35225776F6044513D3E77DBBD6A7A8FE976F506A|GmuF], [protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|Pmi]

    • Structure

  • [PDB|1QWR] ([protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|Pmi], 56% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]-[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]-[gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF]
    description
    [Pubmed|20139185]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20139185], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|F273002AF97D87BB025B4F014C328C5592EAD621|ManR]: activation, in the presence of mannose and absence of glucose [Pubmed|20139185], in [regulon|F273002AF97D87BB025B4F014C328C5592EAD621|ManR regulon]
  • [regulon|RNA switch|RNA switch]: termination/antitermination, expession may be controlled by a potential [SW|RNA switch] located in the 5' untranslated region of the [gene|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]-[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]-[gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF] mRNA between [gene|E26C70893C5D677C816C814558CC42F90B920087|manA] and [gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF], in [regulon|RNA switch|RNA switch]

    • regulation

  • induced by mannose ([protein|search|ManR]) [Pubmed|20139185]
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    Biological materials

    Mutant

  • MGNA-A265 (yjdE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/265 NBRP B. subtilis, Japan]
  • BKE12020 (Δ[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTCGTCATGAAAATCCCCC, downstream forward: _UP4_TAACATGGCAGGGCTTGAGA
  • BKK12020 (Δ[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTCGTCATGAAAATCCCCC, downstream forward: _UP4_TAACATGGCAGGGCTTGAGA

    • References

  • 10960106,20139185,10627040,20862359,23033921,26238998